細胞生産研究チーム

業績

2021

原著論文

Mori Y, Noda S, Shirai T*, Kondo A. (2021) Direct 1,3-butadiene biosynthesis in Escherichia coli via a tailored ferulic acid decarboxylase mutant. Nat Commun. 13;12(1):2195-2206.

Noda S*, Mori Y, Fujiwara R, Shirai T, Tanaka T, Kondo A. (2021) Reprogramming Escherichia coli pyruvate-forming reaction towards chorismate derivatives production. Metab Eng. 67:1-10.

Yuzawa T, Shirai T, Orishimo R, Kawai K, Kondo A, Hirasawa T. (2021) 13C-metabolic flux analysis in glycerol-assimilating strains of Saccharomyces cerevisiae. J Gen Appl Microbiol. in press

Iijima H, Watanabe A, Sukigara H, Iwazumi K, Shirai T, Kondo A, Osanai T. (2021) Four-carbon dicarboxylic acid production through the reductive branch of the open cyanobacterial tricarboxylic acid cycle in Synechocystis sp. PCC 6803. Metab Eng. 65:88-98.

Nonaka D, Fujiwara R, Hirata Y, Tanaka T, Kondo A. (2021) Metabolic engineering of 1,2-propanediol production from cellobiose using beta-glucosidase-expressing E. coli. Bioresour Technol. 329:124858.

2020

原著論文

Fujiwara R, Noda S*, Tanaka T*, Kondo A. Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose-xylose co-substrate. Nat. Commun. 11: 279-290.

Noda S, Mori Y, Oyama S, Kondo A, Araki M, Shirai T*. (2019) Reconstruction of metabolic pathway for isobutanol production in Escherichia coli. Microb. Cell. Fact. 18(1):124-133.

Satowa D, Fujiwara R, Uchio S, Nakano M, Otomo C, Hirata Y, Matsumoto T, Noda S, Tanaka T, Kondo A.(2020) Metabolic engineering of E. coli for improving mevalonate production to promote NADPH regeneration and enhance acetyl-CoA supply. Biotechnol Bioeng. 117(7):2153-2164.

Murai K, Sasaki D, Kobayashi S, Yamaguchi A, Uchikura H, Shirai T, Sasaki K, Kondo A, Tsuge Y. (2020) Optimal Ratio of Carbon Flux between Glycolysis and the Pentose Phosphate Pathway for Amino Acid Accumulation in Corynebacterium glutamicum. ACS Synth Biol. 9(7):1615-1622.

Kobayashi S, Kawaguchi H, Shirai T, Ninomiya K, Takahashi K, Kondo A, Tsuge Y. (2020) Automatic Redirection of Carbon Flux between Glycolysis and Pentose Phosphate Pathway Using an Oxygen-Responsive Metabolic Switch in Corynebacterium glutamicum. ACS Synth Biol. 9(4):814-826.

Iijima H, Watanabe A, Sukigara H, Shirai T, Kondo A, Osanai T. (2020) Simultaneous increases in the levels of compatible solutes by cost-effective cultivation of Synechocystis sp. PCC 6803. Biotechnol Bioeng. 117(6):1649-1660.

2019

原著論文

Sasaki K, Inoue J, Sasaki D, Hoshi N, Shirai T, Fukuda I, Azuma T, Kondo A, Osawa R. (2019) Construction of a Model Culture System of Human Colonic Microbiota to Detect Decreased Lachnospiraceae Abundance and Butyrogenesis in the Feces of Ulcerative Colitis Patients. Biotechnol. J. 14(5):e1800555.
https://www.ncbi.nlm.nih.gov/pubmed/30791234

2018

原著論文

Mori Y, Shirai T*. (2018) Designing artificial metabolic pathways, construction of target enzymes, and analysis of their function. Curr. Opin. Biotechnol. 54:41-44.
https://www.ncbi.nlm.nih.gov/pubmed/29452926

Fujiwara R, Noda S*, Tanaka T*, Kondo A. (2018) Muconic Acid Production Using Gene-Level Fusion Proteins in Escherichia coli. ACS Synth. Biol. 7(11):2698-2705.
https://www.ncbi.nlm.nih.gov/pubmed/30350569

Thomson NM, Shirai T, Chiapello M, Kondo A, Mukherjee KJ, Sivaniah E, Numata K, Summers DK. (2018) Efficient 3-Hydroxybutyrate Production by Quiescent Escherichia coli Microbial Cell Factories is Facilitated by Indole-Induced Proteomic and Metabolomic Changes. Biotechnol. J. 13(5):e1700571.
https://www.ncbi.nlm.nih.gov/pubmed/29266768

2017

原著論文

Noda S, Shirai T, Mori Y, Oyama S, Kondo A. (2017) Engineering a synthetic pathway for maleate in Escherichia coli. Nat. Commun. 8(1):1153-1165.
http://www.ncbi.nlm.nih.gov/pubmed/29074856

Noda S, Kondo A. (2017) Recent Advances in Microbial Production of Aromatic Chemicals and Derivatives. Trends. Biotechnol. 35(8):785-796.
http://www.ncbi.nlm.nih.gov/pubmed/28645530

Teramura H, Sasaki K, Kawaguchi H, Matsuda F, Kikuchi J, Shirai T, Sazuka T, Yamasaki M, Takumi S, Ogino C, Kondo A. (2017) Differences in glucose yield of residues from among varieties of rice, wheat, and sorghum after dilute acid pretreatment. Biosci. Biotechnol. Biochem. 81(8):1650-1656.
http://www.ncbi.nlm.nih.gov/pubmed/28622080

Ueda S, Kawamura Y, Iijima H, Nakajima M, Shirai T, Okamoto M, Kondo A, Hirai MY, Osanai T. (2017) Anionic metabolite biosynthesis enhanced by potassium under dark, anaerobic conditions in cyanobacteria. Sci. Rep. 6:32354-32362.
http://www.ncbi.nlm.nih.gov/pubmed/27576448

2016

原著論文

Noda S, Shirai T, Oyama S, Kondo A. (2016) Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives. Metab. Eng. 33:119-129.
http://www.ncbi.nlm.nih.gov/pubmed/26654797

Shirai T, Osanai T, Kondo A. (2016) Designing intracellular metabolism for production of target compounds by introducing a heterologous metabolic reaction based on a Synechosystis sp. 6803 genome-scale model. Microb. Cell. Fact. 15:13-18.
http://www.ncbi.nlm.nih.gov/pubmed/26783098

Fujiwara R, Noda S, Tanaka T, Kondo A. (2016) Styrene production from a biomass-derived carbon source using a coculture system of phenylalanine ammonia lyase and phenylacrylic acid decarboxylase-expressing Streptomyces lividans transformants. J. Biosci. Bioeng. 122(6):730-735.
http://www.ncbi.nlm.nih.gov/pubmed/27405271

Fujiwara R, Noda S, Kawai Y, Tanaka T, Kondo A. (2016) 4-Vinylphenol production from glucose using recombinant Streptomyces mobaraense expressing a tyrosine ammonia lyase from Rhodobacter sphaeroides. Biotechnol. Lett. 38(9):1543-1549.
http://www.ncbi.nlm.nih.gov/pubmed/27206340

Sasaki K, Okamoto M, Shirai T, Tsuge Y, Fujino A, Sasaki D, Morita M, Matsuda F, Kikuchi J, Kondo A. (2016) Toward the complete utilization of rice straw: Methane fermentation and lignin recovery by a combinational process involving mechanical milling, supporting material and nanofiltration. Bioresour. Technol. 216:830-837.
http://www.ncbi.nlm.nih.gov/pubmed/27318161

Teramura H, Sasaki K, Oshima T, Matsuda F, Okamoto M, Shirai T, Kawaguchi H, Ogino C, Hirano K, Sazuka T, Kitano H, Kikuchi J, Kondo A. (2016) Organosolv pretreatment of sorghum bagasse using a low concentration of hydrophobic solvents such as 1-butanol or 1-pentanol. Biotechnol. Biofuels. 9:27-37.
http://www.ncbi.nlm.nih.gov/pubmed/26839590

2015

原著論文

Osanai T, Shirai T, Iijima H, Nakaya Y, Okamoto M, Kondo A, Hirai MY. (2015) Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium. Front Microbiol. 6,1064.
http://www.ncbi.nlm.nih.gov/pubmed/26500619

Iijima H, Shirai T, Okamoto M, Kondo A, Hirai MY, Osanai T. (2015) Changes in primary metabolism under light and dark conditions in response to overproduction of a response regulator RpaA in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Front Microbiol. 6,888.
http://www.ncbi.nlm.nih.gov/pubmed/26379657

Sasaki K, Okamoto M, Shirai T, Tsuge Y, Teramura H, Sasaki D, Kawaguchi H, Hasunuma T, Ogino C, Matsuda F, Kikuchi J, Kondo A. (2015) Precipitate obtained following membrane separation of hydrothermally pretreated rice straw liquid revealed by 2D NMR to have high lignin content. Biotechnol Biofuels. 8,88.
http://www.ncbi.nlm.nih.gov/pubmed/26101546

Teramura H, Sasaki K, Oshima T, Aikawa S, Matsuda F, Okamoto M, Shirai T, Kawaguchi H, Ogino C, Yamasaki M, Kikuchi J, Kondo A. (2015) Changes in Lignin and Polysaccharide Components in 13 Cultivars of Rice Straw following Dilute Acid Pretreatment as Studied by Solution-State 2D 1H-13C NMR. PLoS One. 10, e0128417.
http://www.ncbi.nlm.nih.gov/pubmed/26083431

Noda, S., Shirai, T., Mochida, K., Matsuda, F., Oyama, S., Okamoto, M., Kondo, A. (2015) Evaluation of Brachypodium distachyon L-tyrosine decarboxylase using L-tyrosine overproduction Saccharomyces cerevisiae., PLoS ONE, 21, e0125488.
http://www.ncbi.nlm.nih.gov/pubmed/25996877

Noda, S., Kawai, Y., Tanaka, T., Kondo, A. (2015) 4-Vinylphenol biosynthesis from cellulose as the sole carbon source using phenolic acid decarboxylase-and tyrosine ammonia lyase-expressing Streptomyces lividans., Bioresource Technology, 180, 59-65.
http://www.ncbi.nlm.nih.gov/pubmed/25585255

Noda, S., Matsumoto, T., Tanaka, T., Kondo, A. (2015) Secretory production of tetrameric native full-length streptavidin with thermostability using Streptomyces lividans as a host., Microbial Cell Factories, 14(1), 5.
http://www.ncbi.nlm.nih.gov/pubmed/25582841

Osanai., T, Shirai., T, Iijima., H, Kuwahara., A, Suzuki., I, Kondo., A, Hirai., MY. (2015) Alteration of cyanobacterial sugar and amino acid metabolism by overexpression hik8, encoding a KaiC-associated histidine kinase. Environ Microbiol, 17, 2430-2340.
http://www.ncbi.nlm.nih.gov/pubmed/25403325

2014

原著論文

Matsuda, F. (2014) Rethinking mass spectrometry-based small molecule identification strategies in metabolomics. Mass Spectrometry. 3(S2), S0038.

Matsuda, F., Ogura, T., Tomita, A., Hirano, I., and Shimizu, H. (2014) NanoLC-MRM based quantitative platform for analyzing multiple enzymes associated with central metabolic pathways of Saccharomyces cerevisiae using ultra-fast mass spectrometry. J. Biosci. Bioeng.. 119, 117-120.
http://www.ncbi.nlm.nih.gov/pubmed/25060728

Joseph, A., Aikawa, S., Sasaki, K., Matsuda, F., Hasunuma, T., and Kondo, A. (2014) Increased biomass production and glycogen accumulation in apcE gene deleted Synechocystis sp. PCC 6803. AMB Express. 4, 17.
http://www.ncbi.nlm.nih.gov/pubmed/24949254

Osanai, T., Oikawa, A., Shirai, T., Kuwahara, A., Iijima, H., Tanaka, K., Ikeuchi, M., Kondo, A., Saito, K. and Hirai, M.Y. (2014) Capillary electrophoresis-mass spectrometry reveals the distribution of carbon metabolites during nitrogen starvation in Synechocystis sp. PCC 6803. 16, 512-524.
http://www.ncbi.nlm.nih.gov/pubmed/23796428

2013

原著論文

Miyazaki, T., Noda, S., Tanaka, T., Kondo, A. (2013) Hyper secretion of Thermobifida fusca beta-glucosidase via a Tat-dependent signal peptide using Streptomyces lividans., Microbial Cell Factories, 12(1), 88
http://www.ncbi.nlm.nih.gov/pubmed/24083334

Kawai, Y., Noda, S., Ogino, C., Takeshima, Y., Okai, N., Tanaka, T., Kondo, A. (2013) p-Hydroxycinnamic acid production directly from cellulose using endoglucanase- and tyrosine ammonia lyase-expressing Streptomyces lividans. Microb. Cell Fact. 12, 45.
http://www.ncbi.nlm.nih.gov/pubmed/23604559

Noda, S., Kawai, Y., Tanaka, T., Kondo, A. (2013) Creation of endoglucanase-secreting Streptomyces lividans for enzyme production using cellulose as the carbon source. Appl. Microbiol. Biotechnol. 97, 5711–5720.
http://www.ncbi.nlm.nih.gov/pubmed/23604559

Noda, S., Miyazaki, T., Tanaka, T., Ogino, C. and Kondo, A. (2013) High-level production of mature active-form Streptomyces mobaraensis transglutaminase via pro-transglutaminase processing using Streptomyces lividans as a host. Biochem. Eng. J. 74: 76-80
http://www.sciencedirect.com/science/article/pii/S1369703X13000508

Matsuda, F., Shirai, T., Ishii, J., Kondo, A. (2013) Regulation of central carbon metabolism in Saccharomyces cerevisiae by metabolic inhibitors, J. Biosci. Bioeng.116, 59-64.
http://www.ncbi.nlm.nih.gov/pubmed/23453202

Shirai, T., Matsuda, F., Okamoto, M., and Kondo, A. (2013) Evaluation of control mechanisms for Saccharomyces cerevisiae central metabolic reactions using metabolome data of eight single-gene deletion mutants, Appl. Microbiol. Biotechnol. 97, 3569-3577.
http://www.ncbi.nlm.nih.gov/pubmed/23224404

2012

原著論文

Ishii, J., Yoshimura, K., Hasunuma, T. and Kondo, A.(2012) Reduction of furan derivatives by overexpressing NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR–XDH pathway, Appl. Microbiol. Biotechnol. 97, 2597-2607.
http://www.ncbi.nlm.nih.gov/pubmed/23001007

Matsuda, F., Kondo, T., Ida, K., Tezuka, H., Ishii, J. and Kondo, A.(2012) Construction of an artificial pathway for isobutanol biosynthesis in the cytosol of Saccharomyces cerevisiae, Biosci. Biotechnol. Biochem. 76, 2139-2141.
http://www.ncbi.nlm.nih.gov/pubmed/23132567

Suga, H., Matsuda, F., Hasunuma, T., Ishii, J., and Kondo, A.(2012) Implementation of a transhydrogenase-like shunt to counter redox imbalance during xylose fermentation in Saccharomyces cerevisiae, Appl. Microbiol. Biotechnol. 97, 1669-1678.
http://www.ncbi.nlm.nih.gov/pubmed/22851014

総　説

Kondo, A., Ishii, J., Hara, K., Hasunuma, T., and Matsuda, F.(2012) Development of microbial cell factories for bio-refinery through synthetic bioengineering, J. Biotech. 163, 204-216.
http://www.ncbi.nlm.nih.gov/pubmed/22728424